Appearance of the Unexpected Triploids in the Hybrid Progeny between Lettuce, Lactuca sativa and its Wild Relatives, L. saligna
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Accession number;04A0254453
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| Title;Appearance of the Unexpected Triploids in the Hybrid Progeny between Lettuce, Lactuca sativa and its Wild Relatives, L. saligna |
| Author;
MIZUTANI T
(Kyushu Tokai Univ., Kumamoto)
TANAKA T
(Kyushu Tokai Univ., Kumamoto)
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Journal Title;Journal of the Japanese Society for Horticultural Science
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Journal Code:F0626A
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ISSN:0013-7626
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VOL.73;NO.2;PAGE.114-118(2004)
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| Figure&Table&Reference;FIG.2, TBL.3, REF.16 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Interspecific hybrids between Lactuca saligna and L. sativa cv. Bronze were obtained only when L. saligna was used as a seed parent. The seed set ratio of the F1 hybrid was very low (12.0%), however, their seed germination rate was 87.5%. In pollen mother cells of the F1 hybrids, univalent chromosomes were observed at metaphase I and the number of chiasma at diakinesis was fewer than their parental species. At pollen tetrad stage, the number of microspores of F1 hybrids varied between 2 and 6. In some F2 hybrids, distorted staining intensities showed a 4:4:1 ratio in all or some of the 5 isozyme loci, suggesting that they were triploids or aneuploids, respectively. The triploids in F2 progeny were screened by flow cytometrie analysis at the 2-week-old seedling stage. Microscopic observation of chromosome revealed that 14 of 103 F2 plants were found to be triploid, 2n=3x=27; no aneuploid plants were observed indicating that aneuploids do not survive until the 3-to 4-week-old seedling stage. (author abst.) |
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