Glycovirus-the size effect on gene delivery via endocytosis.
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Accession number;04A0258597
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| Title;Glycovirus-the size effect on gene delivery via endocytosis. |
| Author;
KANAMORI TAKUYA
(Kyoto Univ., Graduate School, JPN)
HORIUCHI SHOHEI
(Kyoto Univ., Graduate School, JPN)
SANDO SHINSUKE
(Kyoto Univ., Graduate School, JPN)
AOYAMA YASUHIRO
(Kyoto Univ., Graduate School, JPN)
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.18th;NO.;PAGE.28-29(2003)
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| Figure&Table&Reference;FIG.3, REF.3 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Cel8-derived GNPs bind to a 7040 bp plasmid pCMVluc to from what we call glycovirus. The DNA-Cel8 complexation is number-, size- and shape-restricted. The particle sizes were measured by DLS which showed the general order of Mal > Lac >> Cel and Gly8 > Gly5 in size. It is interesting that one alternation of stereochemistry can result in drastic change in the adhesion properties. The size dependence of the transfection efficiencies shown in Figure 3a is understandable in terms of endocytic size limit. The activities are size-regulated; plots of log E vs. dDLS give an excellent linear correlation with a negative slope. Figure 3b shows a similar log E vs. size correlation for hepatic HepG2 cells, which possess receptors recognizing b-galactose residues. As for Cel8, Cel5, Mal8 and Mal5, transfection efficiency is size-controlled as seen in Hela cells. However, Lac8 and Lac5 having b-galactose residues as ligands of the receptors are HepG2-selective, higher (by-102) than expected on the size basis. The size regulation exists in receptor-mediated gene targeting as well. The result of Figure 3b suggests that simple comparison unmodified with size effect is not a good measure to estimate receptor-ligand effect (Lac8/Mal8=1200 while Lac8/Cel8=0.32). (author abst.) |
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