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Accession number;04A0258599
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| Title;Hydroxamate Siderophore Production by the Magnetic Bacterium Magnetospirillum magneticum AMB-1. |
| Author;
CALUGAY R J
(Tokyo Univ. Agriculture And Technol., Tokyo)
OKAMURA Y
(Tokyo Univ. Agriculture And Technol., Tokyo)
WAHYUDI A T
(Tokyo Univ. Agriculture And Technol., Tokyo)
TAKEYAMA H
(Tokyo Univ. Agriculture And Technol., Tokyo)
MATSUNAGA T
(Tokyo Univ. Agriculture And Technol., Tokyo)
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.18th;NO.;PAGE.32-33(2003)
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| Figure&Table&Reference; |
| Pub. Country;Japan |
| Language;English |
| Abstract;Some microorganisms release extracellular high affinity Fe(III)-chelating ligands termed siderophores to sequester Fe(III) under iron deplete conditions. Through successive chromatographic separations, a hydroxamate-like siderophore was obtained from the magnetic bacterium Magnetospirillum magneticum AMB-1. The compound reversed the growth inhibition imposed by the iron chelator nitrilotriacetate (NTA) in wild type but not in mutant cells. The mutation was generated by transposon insertion in a 3792 bp operon which includes a TonB homologue indicating that the hydroxamate transport requires TonB. Exogenously supplied hydroxamate (deferrioxamine) and catechol did not enhance growth of. M. magneticum AMB-1 except citrate which also demonstrated evidence of TonB requirement. Inter- and intraspecies crossfeeding of the endogenous siderophore did not promote growth to other microorganisms. This confers competitive edge for iron acquistion in M. magneticum AMB-1 and indicates structural novelty of the isolated siderophore. This study reports for the first time the evidence of the presence of TonB and specificity of siderophore in the complex iron metabolism of magnetic bacteria. (author abst.) |
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