Development of a Fully Automated SNP Detection System with Nano-Biomagnetic Particles.
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Accession number;04A0258600
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| Title;Development of a Fully Automated SNP Detection System with Nano-Biomagnetic Particles. |
| Author;
MARUYAMA KOHEI
(Tokyo Univ. Agric. and Technol., Fac. Technol., JPN)
NEMOTO ETSUO
(Juki)
TANAKA TSUYOSHI
(Tokyo Univ. Agric. and Technol., Fac. Technol., JPN)
YODA KIYOSHI
(Juki)
KATO TAKAHIKO
(Miyazaki Medical Coll., JPN)
TAKEYAMA HARUKO
(Tokyo Univ. Agric. and Technol., Fac. Technol., JPN)
MATSUNAGA TADASHI
(Tokyo Univ. Agric. and Technol., Fac. Technol., JPN)
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Journal Title;Abstracts. Symposium on Biofunctional Chemistry
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Journal Code:L0836A
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ISSN:
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VOL.18th;NO.;PAGE.34-35(2003)
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| Figure&Table&Reference;FIG.1 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Recently, a semi-automated system for the detection of single nucleotide polymorphism has been developed in TGF-.BETA.1 based on DNA dissociation curve analysis using bio-nano magnetic particles (BMPs). In this study, a fully automated SNP detection system, which includes DNA purification from whole blood, DNA amplification by PCR, SNP detection by hybridization and fluorescence detection using fluorometer, was designed. The system consisted of a platform with a robotic device for the SNP detection, thermal cycler and fluorometer. To evaluate the precision and reliability of SNP detection, the practical use of the platform was evaluated using 1000 samples. The results showed 97% of samples were correctly identified in comparison with the results by the sequencing method. The error (3%) was caused by low amount or low purity of extracted DNA. To complete the fully automated process, DNA purification procedure using dendrimeric magnetic particles was developed. Genomic DNA from whole blood could be tightly bound to the surface of dendrimeric magnetic particles under certain conditions, subsequently the DNA fragment of TGF-.BETA.1 was amplified by PCR reaction. This procedure enables us to develop a fully-automated system by minor optimization of liquid handlings in the platform with a robotic device. (author abst.) |
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