A pyrene-functionalized guanidinium receptor for fluorescence sensing of DNAs and nucleotide derivatives in water.
|
Accession number;04A0258609
|
| Title;A pyrene-functionalized guanidinium receptor for fluorescence sensing of DNAs and nucleotide derivatives in water. |
| Author;
KATO YUICHI
(Tohoku Univ., Grad. Sch.)
NATORI YUTAKA
(Tohoku Univ., Grad. Sch.)
NISHIZAWA SEIICHI
(Tohoku Univ., Grad. Sch.)
TERAMAE NORIO
(Tohoku Univ., Grad. Sch.)
|
Journal Title;Abstracts. Symposium on Biofunctional Chemistry
|
Journal Code:L0836A
|
ISSN:
|
|
VOL.18th;NO.;PAGE.52-53(2003)
|
| Figure&Table&Reference;FIG.2, REF.2 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Use of a pyrene-functionalized guanidinium receptor (Py-Gua) is presented for fluorescence sensing of nucleotide derivatives and DNAs in aquerous media. From the binding studies by 31P NMR and fluorescence spectroscopy, Py-Gua is found to bind to the phosphate groups of ss-DNA (dA16) accompanied by the apperance of pyrene dimer emission. Even though a guanidinium is previously reported to form a very weak 1:1 complex with H2PO4- (<10 M-1) in aqueous solutions, the apparent 1:1 binding constant between Py-Gua and phosphates of ss-DNA, as estimated by fluorescence titration studies, is indeed greater than 6.0*104 M-1, indicating that Py-Gua interacts with ss-DNA in a cooperative fashion, that is , hydrogen bonding with the phosphate groups and stacking with the neighboring nucleobases. On the other hand, Py-Gua binds to ds-DNA (dA16/dT16) by an intercalation between base pairs, and significant quenching of pyrene monomer emission is observed. The results presented here indicate that the difference in the binding fashion of Py-Gua allows the visible fluorescence discrimination between ss- and ds-DNAs. (author abst.) |
|
|
|
Related Articles;
|
|
