Science Links Japan | Fluorescent Response of Polyquinoline Derivatives toward Zinc Ion.

Fluorescent Response of Polyquinoline Derivatives toward Zinc Ion.

Accession number;04A0258610

Title;Fluorescent Response of Polyquinoline Derivatives toward Zinc Ion.

Author; MIKATA YUJI (Narajodai Kyoseise) WAKAMATSU MOTOKO (Nara Women's Univ.) YANO SHIGENOBU (Nara Women's Univ.)

Journal Title;Abstracts. Symposium on Biofunctional Chemistry

Journal Code:L0836A

ISSN:

VOL.18th;NO.;PAGE.54-55(2003)

Figure&Table&Reference;FIG.2

Pub. Country;Japan

Language;Japanese

Abstract;A novel compound, N,N,N',N'-tetrakis(2-quinolylmethyl)ethylenediamine (TQEN) acts as a fluorescent zinc sensor that binds one equivalent of zinc ion. Whereas TQEN in the absence of zinc ion has negligible fluorescence, zinc-bound TQEN shows fluorescene (.LAMBDA.ex=317 nm, .LAMBDA.em=383 nm). The competition experiment revealed that TQEN has less affinity toward zinc ion than N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN), which is well-known cell-permeable zinc chelator. Measured dissociation constant (Kd) of TQEN-Zn complex from curve-fitting of fluorescence titration data in the present experimental condition (DMF/water=1/1) is 6.+-.3 .MU.M. From these results, TQEN can be regarded as a novel platform for new zinc sensor molecules. (author abst.)

Related Articles;