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AMP-dependent nick-sealing of oligonucleotides at high temperature by a thermostable ATP-dependent DNA ligase.

Accession number;04A0258631

Title;AMP-dependent nick-sealing of oligonucleotides at high temperature by a thermostable ATP-dependent DNA ligase.

Author; ATOMI HARUYUKI (Kyoto Univ., Graduate School, JPN) NAKATANI MASARU (Kyoto Univ., Graduate School, JPN) IMANAKA TADAYUKI (Kyoto Univ., Graduate School, JPN)

Journal Title;Abstracts. Symposium on Biofunctional Chemistry

Journal Code:L0836A

ISSN:

VOL.18th;NO.;PAGE.98-99(2003)

Figure&Table&Reference;FIG.1, REF.2

Pub. Country;Japan

Language;Japanese

Abstract;An ATP-dependent DNA ligase from a hyperthermophilic archaeon exhibited an intriguing AMP-dependent nick-sealing activity towards oligonucleotide substrates. Through biochemical analyses, we found that this reaction was mainly due to a change in relative energy levels of substrate and product at different temperatures. (author abst.)

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