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Accession number;04A0436023
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| Title;The maintenance of hepatocyte functions inside microchips |
| Author;
TANAKA YUKI
(Univ. Tokyo, Graduate School, JPN)
SATO KIICHI
(Univ. Tokyo, Graduate School, JPN)
()
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Journal Title;Nippon Kagakkai Koen Yokoshu
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Journal Code:S0493A
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ISSN:0285-7626
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VOL.84th;NO.1;PAGE.11(2004)
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| Figure&Table&Reference;FIG.1 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;A microchip-based cell culture system was developed and a culture of hepatocytes was realized in the system. The microchip was made of glass plates and has a microchannel and a microculture flask inside. Cells suspended in a medium were introduced into the microchip and incubated. Because of a shortage of dissolved oxygen, the cultured cells in the microchip experienced a significant decrease in viability. To overcome it, a continuous medium flow oxygen and nutrition supplying system was designed and constructed. The system realized good cell growth for at least 1 week. Liver-specific functions, such as the synthesis of albumin and urea from hepatocytes were then confirmed. The albumin synthesis and morphology of hepatocytes under various shear stress conditions were examined. Surface treatment conditions of the microchannel wall were also studied. These results are a promising first step towards the goal of a bioreactor. (author abst.) |
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