Science Links Japan | Development of direct analysis of biomolecules in CE-CL detection method and its application to a microchip device.

Development of direct analysis of biomolecules in CE-CL detection method and its application to a microchip device.

Accession number;04A0436024

Title;Development of direct analysis of biomolecules in CE-CL detection method and its application to a microchip device.

Author; SAITO TAKAHIRO (Doshisha Univ., Fac. of Eng.) IKEGAMI KEIICHI (Doshisha Univ., Fac. of Eng.) KAMEDA TAKAHIDE (Doshisha Univ., Fac. of Eng.) TSUKAGOSHI KAZUHIKO (Doshisha Univ., Fac. of Eng.) NAKAJIMA RIICHIRO (Doshisha Univ., Fac. of Eng.)

Journal Title;Nippon Kagakkai Koen Yokoshu

Journal Code:S0493A

ISSN:0285-7626

VOL.84th;NO.1;PAGE.11(2004)

Figure&Table&Reference;

Pub. Country;Japan

Language;Japanese

Abstract;We have developed of direct analysis of biomolecules in CE-CL detection method, in which luminol - hydrogen peroxide - Cu(II) CL reaction was used. A buffer solution including luminol and Cu(II) was used as a migration buffer and hydrogen peroxide solution was added in the CL detection cell. Amino acid samples moved together with luminol and Cu(II) toward the end of capillary and mixed with hydrogen peroxide solution. We examined the effect of hydrogen peroxide concentration in the detection cell on CL peak. Moreover, we tried to apply the present method to microchip device. (author abst.)

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