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Accession number;04A0483481
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| Title;Suppressive Effect of Meloxicam on the Production of Matrix Metalloproteinases and Tissue Inhibitor of Metalloproteinases from Human Synovial Fibroblasts by Lipopolysaccharide Stimulation in vitro |
| Author;
ASANO KAZUHITO
(Showadai I Daiichiseirigaku)
OSHITA YUSUKE
(Showadai I Daiichiseirigaku)
SAKAI MISAKO
(Showadai I Daiichiseirigaku)
O KOEI
(Showadai I Daiichiseirigaku)
MATSUDA TAKAKO
(Showadai I Daiichiseirigaku)
HISAMITSU TADASHI
(Showadai I Daiichiseirigaku)
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Journal Title;Japanese Pharmacology & Therapeutics
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Journal Code:Z0947A
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ISSN:0386-3603
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VOL.32;NO.5;PAGE.273-280(2004)
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| Figure&Table&Reference;FIG.7, REF.18 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Objective: The present study was carried out to examine the influence of meloxicam (MEL) on the production of matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase (TIMP) from human synovial fibroblasts (SF) in vitro. Materials and Methods: SF was induced from synovial tissues obtained from five patients with osteoarthritis at surgical operation. Cells (2 * 104 cells/mL) were stimulated with 1.0 .MU.g/mL lipopolysaccharide (LPS) in the presence of various concentrations of MEL. After 24 h, culture supernatants were collected and MMPs and TIMPs levels were analyzed by ELISA. Results: Addition of MEL at more than 0.7 * 10-6M into cell cultures could suppress MMP-2, MMP-3, and MMP-9 production from SF induced by LPS stimulation. MEL also inhibited production of both TIMP-1 and TIMP-2 from SF, which were increased by LPS stimulation. Conclusion: These results strongly suggest that MEL could suppress joint tissue remodeling through inhibition of MMP production and result in favorable modification of clinical condition of OA. (author abst.) |
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