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Accession number;04A0438535
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| Title;SNP Detection by Mismach-Binding Ligands |
| Author;
NAKATANI KAZUHIKO
(Kyoto Univ., Graduate School, JPN)
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Journal Title;Nippon Kagakkai Koen Yokoshu
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Journal Code:S0493A
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ISSN:0285-7626
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VOL.84th;NO.1;PAGE.A.4(2004)
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| Figure&Table&Reference;FIG.4, REF.3 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;We have synthesized the first SPR sensor that detects cytosine-cytosine (C-C) mismatches in duplex DNA by immobilizing aminonaphthyridine dimer on the gold surface. The ligand consisted of two 2-aminonaphthyridine chromophores and an alkyl linker connecting them strongly stabilized the C-C mismatches regardless to the flanking sequences. The fully matched duplexes were not stabilized at all under the same conditions. The C-T, C-A, and T-T mismatches were also stabilized with a reduced efficiency. SPR analyses of mismatch-containing 27-mer duplexes were performed with the sensor surface on which the aminonaphthyridine dimer was immobilized. (author abst.) |
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