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Accession number;04A0438536
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| Title;Ribonucleopeptides: Design of functional RNA-peptide complexes |
| Author;
MORII TAKASHI
(Kyoto Univ., JPN)
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Journal Title;Nippon Kagakkai Koen Yokoshu
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Journal Code:S0493A
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ISSN:0285-7626
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VOL.84th;NO.1;PAGE.A.4(2004)
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| Figure&Table&Reference;FIG.1, REF.4 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The fluorescent sensor would be an ideal tool for the convenient measurements of intracellular second messengers. We have designed a new class of scaffold for tailor-made receptors, in which a short peptide and RNA with randomized nucleotide region form a stable and specific complex. The randomized nucleotide region was placed next to the peptide binding region of RNA to enable the formation of "ribonucleopeptide (RNP)" pools in the presence of the RNA-binding peptide. In vitro selection of RNA oligonucleotides from the randomized RNP pool afforded RNP receptors specific for ATP. The approach would provide a new strategy for the design of tailor-made RNP receptors. Fluorescent signal transduction is imparted to the RNP reseptor by various fluorophore derivatives. This creates functionalized RNP sensors that show strong fluorescence emission and wavelength changes in the fluorophore. (author abst.) |
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