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Accession number;04A0438540
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| Title;Design Synthesis and Biological Application of Fluorescent Sensor Molecules for Cell Biology |
| Author;
KIKUCHI KAZUYA
(Univ. of Tokyo, Fac. of Pharm. Sci.)
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Journal Title;Nippon Kagakkai Koen Yokoshu
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Journal Code:S0493A
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ISSN:0285-7626
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VOL.84th;NO.1;PAGE.A.6(2004)
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| Figure&Table&Reference;FIG.2, REF.5 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;In this study, we have established the methods for designing ratiometric fluorescent probes based on switching of fluorescence resonance energy transfer (FRET). The changes in donor-acceptor distance or spectral overlap integral between the donor emission and the acceptor absorption were utilized for the FRET switching. Based on these methods, novel ratiometric fluorescent probes for hydrolytic enzymes such as phosphodiesterase and protein tyrosine phosphatase (PTP) have been designed and synthesized. By using our PTP probe, we have succeeded in detecting PTP activity in living cells, and the changes in PTP activity during cell growth was investigated. As a result, the significant increase of PTP activity was detected in the high cell density. (author abst.) |
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