Annexin 5 Inhibits Thyrotropin Releasing Hormone (TRH) Stimulated Prolactin Release in the Primary Culture of Rat Anterior Pituitary Cells
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Accession number;04A0523042
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| Title;Annexin 5 Inhibits Thyrotropin Releasing Hormone (TRH) Stimulated Prolactin Release in the Primary Culture of Rat Anterior Pituitary Cells |
| Author;
KAWAMINAMI M
(Kitasato Univ., Aomori, Jpn)
SENDA A
(Kitasato Univ., Aomori, Jpn)
ETOH S
(Kitasato Univ., Aomori, Jpn)
MIYAOKA H
(Kitasato Univ., Aomori, Jpn)
KURUSU S
(Kitasato Univ., Aomori, Jpn)
HASHIMOTO I
(Kitasato Univ., Aomori, Jpn)
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Journal Title;Endocr J
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Journal Code:F0625A
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ISSN:0918-8959
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VOL.51;NO.3;PAGE.349-354(2004)
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| Figure&Table&Reference;FIG.5, REF.30 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Annexin 5, a novel calcium-phospholipid binding protein, is thought to be involved in hormone secretion by the anterior pituitary gland. Gonadotropin releasing hormone stimulates annexin 5 synthesis, which, in turn, enhances gonadotoropin secretion. On the other hand, annexin 5 was shown to inhibit prolactin release in vitro. To understand the nature of the opposing effects of annexin 5 on these two major pituitary hormones, the present study examines the inhibitory effect of annexin 5 on prolactin release in relation to thyrotropin stimulating hormone (TRH) using primary cultures of anterior pituitary cells of adult female rats. While recombinant rat annexin 5 was found to have little effect on basal prolactin release, it significantly inhibited TRH-stimulated prolactin release. Addition of specific anti-annexin 5 serum to the culture increased basal prolactin release in a concentration dependent manner, and no further increase in prolactin release was observed following application of TRH in the presence of anti-annexin 5. The enhanced basal prolactin release induced by anti-annexin 5 was reversed by the simultaneous administration of indomethacin, an inhibitor of cyclooxygenase. These results demonstrate that endogenous pituitary annexin 5 exerts an inhibitory effect on prolactin release and suggest that this is attained by suppression of eicosanoid synthesis in vitro. (author abst.) |
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