Simplified Method Using Rumen Ammonium Nitrogen as a Blood-test Reagent
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Accession number;04A0543125
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| Title;Simplified Method Using Rumen Ammonium Nitrogen as a Blood-test Reagent |
| Author;
IKUTA KENTARO
(Hyogokennorinsuisangijutsusogose Awajinogise)
NISHIMORI KAZUHIRO
(Gifu Univ., Graduate School, JPN)
OKADA KEIJI
(Iwate Univ., Faculty of Agriculture, JPN)
YASUDA JUN
(Iwate Univ., Faculty of Agriculture, JPN)
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Journal Title;Journal of the Japan Veterinary Medical Association
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Journal Code:Y0220A
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ISSN:0446-6454
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VOL.57;NO.7;PAGE.435-439(2004)
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| Figure&Table&Reference;FIG.4, TBL.1, REF.14 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;As simplified methods of measuring rumen ammonium nitrogen (R-NH3-N), we used the reagent for blood ammonia (NH3-kit), the reagent for serum urea nitrogen (BUN-kit), and the reagent for dry-type blood auto-analyzer (Drychem). The compared measurements made with them and those made with the conventional steam-distillation method. Even when operation error of the sample dilution was included, the coefficient of variance for the Drychem R-NH3-N measurement was 2.86%. This constitutes adequate reproducibility. A significant (P<0.001) correlation (r = 0.978) was observed between Drychem and steam-distillation R-NH3-N measurements. Moreover, the two sets of values approximated each other. Significant (P<0.001) correlation was observed between R-NH3-N measurements obtained with steam-distillation and Drychem R-NH3-N and BUN-kit (r = 0.991) and NH3-kit (r = 0.961) R-NH3-N measurements. But values obtained with NH3-kit were more than 2 times higher than those obtained with Drychem. From these results, we conclude that Drychem and BUN-kit are practical and useful simplified methods of measuring R-NH3-N. (author abst.) |
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