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Accession number;04A0566245
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| Title;Effect of Anesthetics on the Formation of Membrane polypeptides/Pigment Complex in Lipid Bilayers |
| Author;
FURUKAWA MASAYOSHI
(Nagoya Inst. of Technol., Fac. of Eng.)
KAJIWARA NORIAKI
(Nagoya Inst. of Technol., Fac. of Eng.)
IIDA KOJI
(Nagoya Municipal Ind. Res. Inst., JPN)
NANGO MAMORU
(Nagoya Inst. of Technol., Fac. of Eng.)
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Journal Title;Hiroshima Journal of Anesthesia
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Journal Code:S0305A
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ISSN:0385-1664
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VOL.39;NO.3/4;PAGE.91-97(2003)
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| Figure&Table&Reference;FIG.6, TBL.1, REF.22 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The effect of anesthetics on the formation of the light-harvesting polypeptides (LH)/pigment (Zn-Bacteriochlorophyll a; Zn-BChl a) complex incorporated into lipid bilayers was examined to provide insight into a molecular understanding of anesthetics action. The LH/Zn-BChl a complex as a model of membrane-proteins assembly was reconstituted in lipid bilayers of dipalmitoylphosphatidylcholine (DPPC), dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylglycerol (DMPG). The effect of temperature and the addition of halothane on the stability of the LH/Zn-BChl a complex was monitored by the absorbance change of the Qy band of Zn-BChl a in the complex. Reversible structural changes of LH/Zn-BChl a complex in DMPG bilayers were observed between the complex and its denatured monomeric form with increasing the temperature, but not in DMPC and DPPC bilayers. The addition of halothane to the complex in lipid bilayers exhibited pronounced reversible structural change of the complex for all three lipid bilayers, depending on the concentration. The stability of LH/Zn-BChl a complex against halothane was followed in the order of DPPC>DMPC>DMPG, suggesting that the stability depends on the composed lipid species. (author abst.) |
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