Recognition for Long-Chain Fatty Acids by Dipalmitoylphosphatidylcholine Bilayer Membranes
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Accession number;04A0566246
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| Title;Recognition for Long-Chain Fatty Acids by Dipalmitoylphosphatidylcholine Bilayer Membranes |
| Author;
MATSUKI HITOSHI
(Univ. of Tokushima, Fac. of Eng.)
KUSUBE MASATAKA
(Univ. of Tokushima, Fac. of Eng.)
KANESHINA SHOJI
(Univ. of Tokushima, Fac. of Eng.)
KAMAYA HIROSHI
(Univ. Utah School Of Medicine, Utah, Usa)
UEDA ISSAKU
(Univ. Utah School Of Medicine, Utah, Usa)
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Journal Title;Hiroshima Journal of Anesthesia
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Journal Code:S0305A
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ISSN:0385-1664
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VOL.39;NO.3/4;PAGE.99-102(2003)
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| Figure&Table&Reference;FIG.3, TBL.1, REF.6 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The influences of four long-chain fatty acids (palmitic acid, palmitoleic acid, stearic acid, and oleic acid) and a fatty-acid compound (oleamide) on the phase-transition temperatures of dipalmitoylphosphatidylcholine (DPPC) bilayer membranes were examined by differential scanning calorimetric (DSC) measurements. The effect of all fatty acids on the phase transition of DPPC bilayer appeared in the concentration range of .MU.M order and the appearance modes were different in each fatty acid. The pre- and main-transition temperatures of DPPC bilayer membranes were elevated by the addition of fatty acids with a saturated chain. By the addition of fatty acids with an unsaturated chain, the pre-transition temperature was also elevated while the main-transition temperature was conversely depressed. In the case of oleamide, the pre-transition temperature was hardly affected by the addition although the main-transition temperature was depressed. The three differential partition coefficients of the fatty acids between two phases in the DPPC bilayer membranes were evaluated by applying the thermodynamic equation to the variation of phase-transition temperatures. The results of differential partition coefficients and transition enthalpies showed that the fatty acids with a saturated chain have an affinity for gel phase and decrease the fluidity of DPPC bilayers, whereas those with an unsaturated chain have an affinity for liquid-crystalline phase and increase the fluidity to the contrary. Present study clearly implies that DPPC bilayer membranes have high receptivity for ligand molecules and are able to recognize the long-chain fatty acids. (author abst.) |
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