An Evaluation Method of Bacterial Flora in Contaminated Soils-For the Purpose of a Countermeasure against H2S Gas Production-
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Accession number;04A0659902
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| Title;An Evaluation Method of Bacterial Flora in Contaminated Soils-For the Purpose of a Countermeasure against H2S Gas Production- |
| Author;
TANIGUCHI HATSUMI
(Univ. Occupational and Environmental Health, School of Medicine, Dep. Microbiology, JPN)
FUKUDA KAZUMASA
(Univ. Occupational and Environmental Health, School of Medicine, Dep. Microbiology, JPN)
YAN W
(Univ. Occupational and Environmental Health, School of Medicine, Dep. Microbiology, JPN)
YAMAUCHI KAZUKI
(Univ. Occupational and Environmental Health, School of Medicine, Dep. Microbiology, JPN)
MIZUNO KOHEI
(Kitakyushu National Coll. Technol., JPN)
ISHIMATSU SUMIYO
(Univ. Occupational and Environmental Health, School of Health Sci., JPN)
SERA NOBUYUKI
(Fukuoka Inst. Health and Environmental Sci.)
HAMASAKI MITSUHIRO
(Fukuoka Inst. Health and Environmental Sci.)
TAKAHASHI KOJI
(Fukuoka Inst. Health and Environmental Sci.)
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Journal Title;J UOEH Occup Environ Health
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Journal Code:Z0840A
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ISSN:0387-821X
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VOL.26;NO.3;PAGE.349-367(2004)
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| Figure&Table&Reference;FIG.3, TBL.5, REF.14 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Gas productions in illegal dumping sites and waste landfills have caused serious problems. The gas production was induced by bacterial flora inhabited soils. In order to construct a culture independent evaluation system of the soil bacteria, bacterial communities were analyzed quantitatively and qualitatively, about 16 soil samples at 4 sites, both using culture and culture-independent methods. The real time PCR method was developed for counting total bacterial number. Sequencing analysis of 16S rDNA amplified by a direct PCR method revealed that non-spore forming sulfate reducing bacteria and sulfur-oxidizing bacteria were detected at a similar frequency at an illegal dumping site near mountains. On the other hand, sporeforming sulfate reducing bacteria and Clostridium sp. were mainly detected in deep samples at reclaimed landfills from the sea, whereas sulfur-oxidizing bacteria was hardly detected. The result regarding sulfate-reducing bacteria was confirmed also by an anaerobic culture method. Culture-independent molecular analyses of soil bacteria would give us useful information for prediction of gas production and for the evaluation of soil equilibrium. (author abst.) |
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