Influence of Epinastine Hydrochloride on the Production of Eosinophil Chemo-attractants, RANTES and Eotaxin, in vitro and in vivo
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Accession number;04A0774349
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| Title;Influence of Epinastine Hydrochloride on the Production of Eosinophil Chemo-attractants, RANTES and Eotaxin, in vitro and in vivo |
| Author;
ASANO KAZUHITO
(Showadai I Daiichiseirigaku)
INAGAKI MIKIYA
(School of Medicine, Showa Univl., JPN)
KANAI KEN'ICHI
(School of Medicine, Showa Univl., JPN)
SUZAKI HARUMI
(School of Medicine, Showa Univl., JPN)
HISAMITSU TADASHI
(Showadai I Daiichiseirigaku)
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Journal Title;Japanese Pharmacology & Therapeutics
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Journal Code:Z0947A
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ISSN:0386-3603
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VOL.32;NO.9;PAGE.561-567(2004)
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| Figure&Table&Reference;FIG.3, TBL.2, REF.17 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Objective: The influence of epinastine hydrochloride (EP) on the production of RANTES and eotaxin from nasal fibroblasts was firstly examined in vitro. In the second part of the experiments, we also examined the influence of EP on the appearance of RANTES and eotaxin in nasal secretion obtained from pollinosis patients treated with EP during Japanese cedar pollen season. Methods: Nasal fibroblasts derived from nasal polyps (NF) at 1 * 105 cells/mL were stimulated with 1.0 .MU.g/mL lipopolysaccharide (LPS) in the presence of various concentrations of EP for 24h. RANTES and eotaxin contents in culture supernatants were examined by ELISA. The levels of both RANTES and eotaxin in nasal secretion were also examined by ELISA. Results: Addition of EP at more than 50.0 ng/mL into cell cultures significantly suppressed the production of both RANTES and eotaxin from NF in response to LPS stimulation. Oral administration of EP into pollinosis subjects for two to three weeks slightly inhibited the appearance of RANTES in nasal secretion. On the other hand, the appearance of eotaxin in nasal secretion was clearly suppressed by EP treatment all nasal secretions from subjects treated with EP for two to three weeks contained significant low levels of eotaxin as compared with those from pre-treated subjects. Conclusion: These results strongly suggest that EP modulates the ability of nasal cells, including fibroblasts to produce RANTES and eotaxin, and results in favolable modification of clinical conditions in allergic diseases, especially pollinosis. (author abst.) |
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