Effects of Calcium Regulating Hormones on Osteoclast-Like Cell Formation in Hen Medullary Bone Marrow Culture
|
Accession number;04A0805721
|
| Title;Effects of Calcium Regulating Hormones on Osteoclast-Like Cell Formation in Hen Medullary Bone Marrow Culture |
| Author;
SUGIYAMA T
(Niigata Univ., Niigata, Jpn)
SAKURAI M
(Niigata Univ., Niigata, Jpn)
HIYAMA S
(Niigata Univ., Niigata, Jpn)
KUSUHARA S
(Niigata Univ., Niigata, Jpn)
|
Journal Title;J Poult Sci
|
Journal Code:G0008B
|
ISSN:1346-7395
|
|
VOL.41;NO.4;PAGE.307-314(2004)
|
| Figure&Table&Reference;FIG.4, REF.37 |
| Pub. Country;Japan |
| Language;English |
| Abstract;In order to examine the effects of the calcium regulating hormones on the differentiation of hen medullary bone osteoclasts, isolated medullary bone marrow cells were cultured for 8 days in media including parathyroid hormone (PTH), 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] or 17/.BETA.-estradiol (E2), respectively. After 8 days of culture, tartrate-resistant acid phosphatase (TRAP) activity, a marker for osteoclasts, was enzymehistochemically detected on the cultured marrow cells and these cells were also stained with Mayer's hematoxylin. Thereafter, TRAP-positive multinuclear cells were counted as osteoclast-like cells, and the effects of the calcium regulating hormones on the osteoclast differentiation from medullary bone marrow cells were indicated as the percentage (%) of the controls when vehicle was added in place of the hormones. As a result, treatment with PTH or 1,25(OH)2D3 significantly stimulated the differentiation of osteoclast-like cells, up to 218.3% or 159.2%, respectively. On the other hand, E2 treatment resulted in a moderate, but not significant, suppression of differentiation (85.8% of controls). These results indicate that PTH and 1,25(OH)2D3 stimulate medullary bone osteoclast differentiation, but E2 is likely inhibitory. (author abst.) |
|
|
|
Related Articles;
|
|
|
