Comparative Studies on Levels of Proteins, Bacterial Endotoxins and Nucleic Acids in Hyaluronan Preparations Used to Treat Osteoarthritis of the Knee-Could Residual Proteins and Bacterial Endotoxins Relate to Complications?-
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Accession number;04A0864557
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| Title;Comparative Studies on Levels of Proteins, Bacterial Endotoxins and Nucleic Acids in Hyaluronan Preparations Used to Treat Osteoarthritis of the Knee-Could Residual Proteins and Bacterial Endotoxins Relate to Complications?- |
| Author;
OHSHIMA Y
(Hatano Res. Inst.)
YOKOTA S
(Hatano Res. Inst.)
KASAMA K
(Hatano Res. Inst.)
ONO H
(Hatano Res. Inst.)
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Journal Title;Japanese Pharmacology & Therapeutics
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Journal Code:Z0947A
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ISSN:0386-3603
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VOL.32;NO.10;PAGE.655-662(2004)
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| Figure&Table&Reference;FIG.2, TBL.1, REF.39 |
| Pub. Country;Japan |
| Language;English |
| Abstract;The levels of proteins, bacterial endotoxins and absorbance of 260 nm related to deoxyribonucleic acids (DNA) were measured in several hyaluronan (HA) preparations used for the treatment of knee osteoarthritis (OA): Hyalgan, Artz and Synvisc. Hyalgan and Artz are classified as non-modified HA preparations, while Synvisc is classified as a modified HA preparation in which the HA chains are cross-linked, utilizing proteins derived from chicken combs. Therefore, Synvisc was included relatively large amount of residual proteins in comparison with that of other HA preparations. The residual protein contents in Hyalgan, Artz and Synvisc were 4.5.+-.0.6 .MU.g/mL, 0.5.+-.0.1 .MU.g/mL and 7.5.+-.1.8 .MU.g/mL, respectively. The contaminant levels of bacterial endotoxins in Hyalgan, Artz and Synvisc were 0.0074.+-.0.0026 EU/mL, 0.0045.+-.0.0001 EU/mL and 0.19915.+-.0.0741 EU/mL. Spectrophotometric analyses (absorbance at 260 nm) in Hyalgan, Artz and Synvisc were 0.107.+-.0.004, 0.026.+-.0.003 and 0.167.+-.0.073. These results indicated that Synvisc included quite high content of parameters such as bacterial endotoxins, proteins in comparison with non-modified HA preparation; Hyalgan and Artz. (author abst.) |
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