Influence of Roxithromycin and Telithromycin on Cytokine Production from Human Peripheral Blood CD4 + T Cells in vitro
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Accession number;04A0864559
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| Title;Influence of Roxithromycin and Telithromycin on Cytokine Production from Human Peripheral Blood CD4 + T Cells in vitro |
| Author;
KYO YOSHIYUKI
(School of Medicine, Showa Univl., JPN)
KANAI KEN'ICHI
(School of Medicine, Showa Univl., JPN)
ASANO KAZUHITO
(Showadai I Daiichiseirigaku)
HISAMITSU TADASHI
(Showadai I Daiichiseirigaku)
SUZAKI HARUMI
(School of Medicine, Showa Univl., JPN)
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Journal Title;Japanese Pharmacology & Therapeutics
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Journal Code:Z0947A
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ISSN:0386-3603
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VOL.32;NO.10;PAGE.679-684(2004)
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| Figure&Table&Reference;FIG.5, REF.21 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Objective: The present study was undertaken to examine the influence of roxithromycin (RXM) and telithromycin (TEL) on the production of T-cell cytokines from human peripheral blood CD4 + T cells under stimulation with co-stimulatory molecules. Materials and Methods: CD4 + T cells from healthy donors were cultured in the presence of various concentrations of either RXM or TEL on OKT-3 and anti-CD28 monoclonal antibody-coated wells. T cell proliferation, along with the concentrations of interleukin (IL)-2, interferon (IFN)-.GAMMA., IL-4 and IL-5 were measured. Results: RXM and TEL did not affect T-cell proliferation by co-stimulatory stimulation as assessed by 3H-thymidine incorporation. Although RXM did not inhibit the production of IL-2 and IFN-.GAMMA., the ability of cells to produce IL-4 and IL-5 was suppressed in dose-dependent manner, when the agent was added to cell cultures at more than 5.0 .MU.g/mL. On the other hand, TEL could not suppress the production of IL-4 and IL-5 in response to co-stimulatory molecule stimulation, even when 2.0 .MU.g/mL, twice a therapeutic blood level, of TEL was added to cell cultures. Conclusion: These results strongly suggests that RXM modulates the clinical condition of chronic inflammatory airway diseases through the suppression of IL-4 and IL-5 production. It is also indicated that TEL may not have novel anti-inflammatory actions. (author abst.) |
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