Rho Regulates Hepatic Sinusoidal Endothelial Fenestral Actin Cytoskeleton
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Accession number;05A0094901
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| Title;Rho Regulates Hepatic Sinusoidal Endothelial Fenestral Actin Cytoskeleton |
| Author;
YOKOMORI HIROAKI
(Kitasato Inst., Medical Cent. Hosp.)
ODA MASAYA
(International Univ. Health and Welfare, JPN)
HIBI TOSHIFUMI
(Keio Univ.)
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Journal Title;Japanese Pharmacology & Therapeutics
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Journal Code:Z0947A
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ISSN:0386-3603
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VOL.32;NO.Suppl.2;PAGE.S.149-S.150(2004)
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| Figure&Table&Reference;FIG.1, REF.3 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The presence of microfilaments in the vicinity of sinusoidal endothelial fenestrae (SEF) indicates that the cytoskeleton of sinusoidal endothelial cells (SEC) plays an important role in the modulation of SEF. Rho has emerged as an important regulator of the actin cytoskeleton, and consequently cell morphology. The present study aimed to examine how a Rho stimulator; lysophophatidic acid (LPA), and a Rho inhibitor; bacterial toxin C3 transferase (C3-transferase), affect the morphology of SEF. Monolayers of SEC culture established by collagenase infusion of a rat liver were incubated for 24 hours. The cells were separated into three groups; control, LPA-treated, and C3-transferase-treated groups. SEF morphology was observed by scanning electron microscopy. Formation of F-actin stress fibers was observed by confocal microscopy. Treatment of SECs with LPA contracted the SEF, concomitant with increases in F-actin stress fiber and actin microfilament. Following treatment with C3-transferase, SEF dilated and fused, concomitant with a loss of F-actin and microfilament. In conclusion, these results indicate that Rho regulates fenestral changes in SEC via regulation of the actin cytoskeleton. (author abst.) |
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