Separation of 2-Aminobenzoic Acid-Derivatized Glycosaminoglycans and Asparagine-linked Glycans by Capillary Electrophoresis
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Accession number;05A0095061
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| Title;Separation of 2-Aminobenzoic Acid-Derivatized Glycosaminoglycans and Asparagine-linked Glycans by Capillary Electrophoresis |
| Author;
SATO K
(Univ. Tokyo, Tokyo, Jpn)
SATO K
(Univ. Tokyo, Tokyo, Jpn)
OKUBO A
(Univ. Tokyo, Tokyo, Jpn)
YAMAZAKI S
(Nagasaki Univ., Nagasaki, Jpn)
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Journal Title;Anal Sci
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Journal Code:G0673B
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ISSN:0910-6340
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VOL.21;NO.1;PAGE.21-24(2005)
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| Figure&Table&Reference;FIG.6, REF.20 |
| Pub. Country;Japan |
| Language;English |
| Abstract;A capillary electrophoresis method was developed for the analysis of oligosaccharides combined with derivatization with 2-aminobenzoic acid. Glycosaminoglycan .DELTA.-disaccharides were effectively resolved on a fused-silica capillary tube using 150 mM borate, pH 8.5, as a running electrolyte solution. This analytical method was applied to the identification of glycosaminoglycan in combination with enzymatic digestion. The separation of N-glycans or glucose-oligomers was performed with a phosphate buffer containing polyethylene glycol or borate as an electrolyte solution. This method is expected to be useful in the determination of oligosaccharide structures in a glycoprotein. (author abst.) |
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