Chiral Separation of NBD-Amino Acids by Ligand-Exchange Micro-Channel Electrophoresis
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Accession number;05A0095070
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| Title;Chiral Separation of NBD-Amino Acids by Ligand-Exchange Micro-Channel Electrophoresis |
| Author;
NAKAJIMA H
(Tokyo Metropolitan Univ., Tokyo, Jpn)
KAWATA K
(Tokyo Metropolitan Univ., Tokyo, Jpn)
SHEN H
(Tokyo Metropolitan Univ., Tokyo, Jpn)
NAKAGAMA T
(Tokyo Metropolitan Univ., Tokyo, Jpn)
UCHIYAMA K
(Tokyo Metropolitan Univ., Tokyo, Jpn)
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Journal Title;Anal Sci
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Journal Code:G0673B
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ISSN:0910-6340
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VOL.21;NO.1;PAGE.67-71(2005)
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| Figure&Table&Reference;FIG.7, TBL.1, REF.40 |
| Pub. Country;Japan |
| Language;English |
| Abstract;The chiral separation of amino acid derivatives by ligand-exchange electrophoresis in a microchannel chip was performed for the first time. A Cu(II) complex with L-prolinamide was used as a chiral selector. The migration behaviors of eleven NBD-DL-amino acids were investigated by ligand-exchange capillary electrophoresis (LE-CE). The enantiomer of five NBD-amino acids (Ser, Thr, Val, Phe and His) could be separated by LE-CE using a 20 mM ammonium acetate buffer (pH 9.0) containing 10 mM copper acetate, 20 mM L-prolinamide and 1 mM SDS. NBD-His was eluted in the order D-form and L-form, while the elution order of another enantiomers was L-form and D-form. Under this condition, the enantioseparation of these five NBD-amino acids by ligand-exchange microchip electrophoresis (LE-ME) was investigated using a glass microchip. The enantioseparation of NBD-Ser, -Thr and -His could be successfully accomplished by LE-ME. LE-ME was superior to LE-CE in terms of the short migration time and a good enantiomeric separation. (author abst.) |
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