Chiral CE Separation of Dopamine-Derived Neurotoxins
|
Accession number;05A0184727
|
| Title;Chiral CE Separation of Dopamine-Derived Neurotoxins |
| Author;
QUAN Z
(Jackson State Univ., Ms, Usa)
SONG Y
(Jackson State Univ., Ms, Usa)
PETERS G
(Jackson State Univ., Ms, Usa)
SHENWU M
(Jackson State Univ., Ms, Usa)
SHENG Y
(Jackson State Univ., Ms, Usa)
HWANG H-M
(Jackson State Univ., Ms, Usa)
LIU Y-M
(Jackson State Univ., Ms, Usa)
|
Journal Title;Anal Sci
|
Journal Code:G0673B
|
ISSN:0910-6340
|
|
VOL.21;NO.2;PAGE.115-119(2005)
|
| Figure&Table&Reference;FIG.5, TBL.1, REF.34 |
| Pub. Country;Japan |
| Language;English |
| Abstract;An enantiomeric separation of dopamine-derived neurotoxins by capillary electrophoresis has been developed. Tetrahydroisoquinoline (TIQ), dopamine (DA), (R/S)-1-benzyl-TIQ (BTIQ), (R/S)-6,7-dihydroxy-1-methyl-TIQ (salsolinol, Sal), and (R/S)-6,7-dihydroxy-1, 2-dimethyl-TIQ (N-methyl-salsolinol, NMSal) were studied as model compounds. The CE running buffer (50 mM phosphate buffer at pH 3.0) contained 1.5 M urea and 12 mM .BETA.-CD as a chiral selector. During separation, the (R)-enantiomers formed more stable inclusion complexes with .BETA.-CD, and thus had a longer migration time than their optical antipodes. It was noticed that the recovery rates of these TIQ derivatives were very poor (< 15%) during protein precipitation, a procedure widely used for cleaning up biological samples. The recovery was significantly improved by pre-mixing the sample with a surfactant (e.g., sodium hexanesulfonate or Triton X-100) to reduce the co-precipitation. The present method in combination with electrospray ionization tandem mass spectrometry (ESI-MS/MS) was applied to study samples obtained from in vitro incubation of two catecholamines, dopamine and epinine, with aldehydes forming neurotoxins including (S)- and (R)-NMSal enantiomers. The later is known to induce Parkinsonism in rats. (author abst.) |
|
|
|
Related Articles;
|
|
|
