Detection Limit Estimated from Slope of Calibration Curve: An Application to Competitive ELISA
|
Accession number;05A0184736
|
| Title;Detection Limit Estimated from Slope of Calibration Curve: An Application to Competitive ELISA |
| Author;
HAYASHI Y
(National Inst. Of Health Sci., Tokyo, Jpn)
MATSUDA R
(National Inst. Of Health Sci., Tokyo, Jpn)
ITO K
(Showa Univ., Tokyo, Jpn)
NISHIMURA W
(Showa Univ., Tokyo, Jpn)
IMAI K
(Musashino Univ., Nishitokyo, Jpn)
MAEDA M
(National Inst. Of Health Sci., Tokyo, Jpn)
|
Journal Title;Anal Sci
|
Journal Code:G0673B
|
ISSN:0910-6340
|
|
VOL.21;NO.2;PAGE.167-169(2005)
|
| Figure&Table&Reference;FIG.1, REF.9 |
| Pub. Country;Japan |
| Language;English |
| Abstract;This paper theoretically derives a general rule that while the slope of the semi-logarithmic plot (Y vs. log X) of a calibration curve varies depending on analyte concentration, X, the slope takes a specific value at the detection limit (LD). This rule holds good irrespective of the shape of the calibration curve (linear or non-linear) and in this paper, is applied to competitive ELISA (enzyme linked immunosorbent assay). The following relationship is deduced: slope of log-dose B/B0 at LD = [relative standard deviation (RSD) of blank responses] / 0.13. The LD obtained from the above-mentioned slope corresponds to the dose at which the RSD of dose estimates is 0.3 (= 30%). A commercial kit for 17.ALPHA.-hydroxyprogesterone is taken as an example. (author abst.) |
|
|
|
Related Articles;
|
|
|
