Preparation of pathological samples Artifact in staining.
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Accession number;05A0136087
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| Title;Preparation of pathological samples Artifact in staining. |
| Author;
SUEYOSHI NORIYOSHI
(Juntendodai Daigakuin'igakukenkyuka Kenkyukisose Saiboubyoriimejingukenkyubumon)
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Journal Title;Modern Medical Laboratory
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Journal Code:Z0084B
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ISSN:0301-2611
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VOL.33;NO.1;PAGE.70-73(2005)
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| Figure&Table&Reference;FIG.7, TBL.1 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The cause of artifact in staining and the countermeasures are described. When a part of the image is dark and clearly with a different refractive index from other parts, some other substances than the encapsulant, such as water, may be included in the dark part. As a countermeasure, it is necessary to pay attention to the purity of the alcohol used in the dehydration. Also, when it is transferred into different solvents, the work is performed while confirming that the whole sample is surely in affinity with each solvent. When the image is of a poor quality, generally, and could not be focused by a high magnification lens, plural pieces of cover glass may be used in piles. When a lipid droplet is to be proven, the surrounding tissue is fixed in the fixative which does not dissolve the lipid droplet to retain the lipid droplet until dyeing. Slicing of unfixed fat tissue is usually performed at below -30.DEG.C. (for formalin-fixed tissue, around -15.DEG.C.). |
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