MODIFIED NEAREST NEIGHBOR METHOD FOR 3D IMAGING IN FLUORESCENCE MICROSCOPY
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Accession number;05A0212964
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| Title;MODIFIED NEAREST NEIGHBOR METHOD FOR 3D IMAGING IN FLUORESCENCE MICROSCOPY |
| Author;
KOJI Y
(Tokyo Inst. Technol., Yokohama, Jpn)
KUMAZAWA I
(Tokyo Inst. Technol., Yokohama, Jpn)
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Journal Title;IEIC Technical Report (Institute of Electronics, Information and Communication Engineers)
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Journal Code:S0532B
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ISSN:0913-5685
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VOL.104;NO.544(IE2004 124-144);PAGE.85-90(2005)
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| Figure&Table&Reference;FIG.12, REF.8 |
| Pub. Country;Japan |
| Language;English |
| Abstract;The fluorescence microscopy is suitable to observe a plane surface of an object but, when it comes to observe a three-dimensional structure of an object, light emissions from structures at different depth are mixed and it is difficult to isolate a section image on a target plane. Various techniques have been proposed to solve this problem but current techniques require an unaffordable computation cost and simplified techniques to save the cost, such as the nearest neighbor method, only produce low quality images. In this paper, we propose a technique that separates the out-of-focus-effect from front planes and that from behind planes and, improves the accuracy in separating the section image on the target plane. We evaluated effectiveness of this technique through some experiments and showed improved results under reduced computational cost. (author abst.) |
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