Noninvasive Detection of Tissue Remodeling After Acute Myocardial Infarction in Rats by Indium-111-Anti-Tenascin-C Antibody and SPECT
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Accession number;05A0202011
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| Title;Noninvasive Detection of Tissue Remodeling After Acute Myocardial Infarction in Rats by Indium-111-Anti-Tenascin-C Antibody and SPECT |
| Author;
ODAKA KEN'ICHI
(Graduate School of Medicine, Chiba Univ., JPN)
UEHARA TOMOYA
(Graduate School of Pharmaceutical Sci., Chiba Univ., JPN)
ARANO YASUSHI
(Graduate School of Pharmaceutical Sci., Chiba Univ., JPN)
TADOKORO HIROYUKI
(Graduate School of Medicine, Chiba Univ., JPN)
IMANAKA(YOSHIDA) KYOKO
(Mie Univ., School of Medicine, JPN)
YOSHIDA TOSHIMICHI
(Mie Univ., School of Medicine, JPN)
HIROE MICHIAKI
(Kokuritsukokusaiiryose Jinzojunkankika)
IRIE TOSHIAKI
(National Inst. Radiological Sciences, JPN)
TANADA SHUJI
(National Inst. Radiological Sciences, JPN)
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Journal Title;J Cardiol
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Journal Code:Y0264A
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ISSN:0914-5087
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VOL.45;NO.2;PAGE.90-91(2005)
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| Figure&Table&Reference;REF.2 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Background. Left ventricular remodeling after acute myocardial infarction causes heart failure and poor prognosis, and thus it is important to evaluate the degree of the remodeling. Expression of tenascin-C (TNC), one of the extracellular matrix glycoproteins, is rapidly and abundantly induced in the left ventricle after myocardial infarction. We developed a new noninvasive diagnostic technique to detect the tissue remodeling after acute myocardial infarction using radiolabeled anti-TNC antibody. Methods and Results. 111In-anti-TNC monoclonal antibody was injected intravenously at 1 day (D1, n=3), 3 days (D3, n=5), and 5 days (D5, n=5) after producing myocardial infarction and 5 sham-operated rats. The radioactivity in the heart was significantly higher in D1, D3, and D5 (D1: 0.45.+-.0.06, D3: 0.64.+-.0.12, D5: 0.38.+-.0.07% injected-dose/g) than sham-operated rats (0.27.+-.0.06, p<0.01 vs D1 and D3, p<0.05 vs D5). By autoradiography, high radioactivities were observed in the granulation tissue around the necrotic area of infarcted hearts. Dual-isotope single-photon emission computed tomography imaging of 111In-anti-TNC antibody and 99mTc-methoxy-isobutyl isonitrile demonstrated the regional myocardial uptake of 111In-anti-TNC antibody, which was complementary to the perfusion image. Conclusions. The present study indicated that we can localize the infarcted region in the heart by ex vivo and in vivo imagings using the radiolabeled anti-TNC fragments, suggesting its potential usefulness for noninvasive detection of left ventricular remodeling. (author abst.) |
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