Interaction Analysis of the Carcinoembryonic Antigen (CEA) with Its Monoclonal Antibody Immobilized on a Gold Surface Using Fourier Transform Infrared Reflection-Absorption Spectroscopy (FT-IR RAS)
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Accession number;05A0362054
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| Title;Interaction Analysis of the Carcinoembryonic Antigen (CEA) with Its Monoclonal Antibody Immobilized on a Gold Surface Using Fourier Transform Infrared Reflection-Absorption Spectroscopy (FT-IR RAS) |
| Author;
OHTSUKA KEIICHI
(Kyushu Univ., Fukuoka, Jpn)
KUROKI MASAHIDE
(Fukuoka Univ., Fukuoka, Jpn)
NOJIMA TAKAHIKO
(Kyushu Univ., Fukuoka, Jpn)
WAKI MICHINORI
(Kyushu Univ., Fukuoka, Jpn)
TAKENAKA SHIGEORI
(Kyushu Univ., Fukuoka, Jpn)
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Journal Title;Anal Sci
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Journal Code:G0673B
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ISSN:0910-6340
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VOL.21;NO.3;PAGE.215-218(2005)
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| Figure&Table&Reference;FIG.5, REF.17 |
| Pub. Country;Japan |
| Language;English |
| Abstract;A monoclonal antibody for the carcinoembryonic antigen (CEA) was immobilized on a gold chip surface covered by a self-assembled monolayer of 11-mercaptoundecanoic acid. Upon the addition of CEA, a Fourier transform infrared reflection-absorption spectroscopy (FT-IR RAS) measurement showed an increased absorption at around 1500-1700 cm-1, corresponding to its amide structures. Another addition of CEA polyclonal antibody on this chip caused a further increase of the absorption in this region only after a treatment with CEA. This result shows that an antibody-fixed gold surface coupled with an FT-IR RAS measurement provides a new tool for detecting the antibody-antigen interaction. (author abst.) |
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