Continuous in vivo Monitoring of Blood Diffusible Calcium Using On-line Microdialysis Sampling Coupled with Flame Atomic Absorption Spectrometry
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Accession number;05A0362056
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| Title;Continuous in vivo Monitoring of Blood Diffusible Calcium Using On-line Microdialysis Sampling Coupled with Flame Atomic Absorption Spectrometry |
| Author;
TSENG WEI-CHANG
(Fooyin Univ., Kaohsiung, Twn)
SUN YUH-CHANG
(National Tsing-hua Univ., Hsinchu, Twn)
LEE CHENG-FA
(Kaohsiung Medical Univ., Kaohsiung, Twn)
YANG MO-HSIUNG
(National Tsing-hua Univ., Hsinchu, Twn)
HUANG YEOU-LIH
(Kaohsiung Medical Univ., Kaohsiung, Twn)
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Journal Title;Anal Sci
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Journal Code:G0673B
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ISSN:0910-6340
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VOL.21;NO.3;PAGE.225-229(2005)
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| Figure&Table&Reference;FIG.5, TBL.2, REF.24 |
| Pub. Country;Japan |
| Language;English |
| Abstract;A direct, rapid and continuous in vivo monitoring of diffusible calcium in the blood of living rabbits has been developed using microdialysis sampling coupled on-line with flame atomic absorption spectrometry. Microdialysates perfused through implanted microdialysis probes were collected with a sample loop on an injection valve and directly introduced into the flame atomizer by a carrier solution. An ultrapure saline solution (0.9% NaCl, pH 7.2) was used as the perfusion solution at a flow rate of 20 .MU.l min-1 via the microdialysis probe. A 0.1% La solution in 0.5% HNO3 solution was employed as the carrier solution at a nebulizer uptake flow rate of 2.5 ml min-1. The interval for each determination was 2.5 min (2 min of sampling time, 20 s of read time and 10 s of washing time). The performance characteristics of the on-line microdialysis-FAAS system were validated as follows: linearity range, 0-100 mg l-1; detection limit (3 .SIGMA., n = 7), 3.66 mg l-1; precision (RSD, n = 50), 6.2%. For the evaluation of analytical accuracy, the proposed on-line method was compared with the in vivo no net flux method. The use of an on-line microdialysis-FAAS system permitted the in situ, dynamic and continuous in vivo monitoring of diffusible calcium in the blood of the living rabbits after CaCl2 administration with a temporal resolution of 2.5 min. (author abst.) |
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