Analysis of Tetracycline Antibiotics Using HPLC with Pulsed Amperometric Detection
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Accession number;05A0362059
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| Title;Analysis of Tetracycline Antibiotics Using HPLC with Pulsed Amperometric Detection |
| Author;
CHAROENRAKS THIRAPORN
(Chulalongkorn Univ., Bangkok, Tha)
CHUANUWATANAKUL SUCHADA
(Chulalongkorn Univ., Bangkok, Tha)
HONDA KENSUKE
(Yamaguchi Univ., Yamaguchi, Jpn)
YAMAGUCHI YOKO
(Yamaguchi Univ., Yamaguchi, Jpn)
CHAILAPAKUL ORAWON
(Chulalongkorn Univ., Bangkok, Tha)
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Journal Title;Anal Sci
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Journal Code:G0673B
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ISSN:0910-6340
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VOL.21;NO.3;PAGE.241-245(2005)
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| Figure&Table&Reference;FIG.4, TBL.3, REF.33 |
| Pub. Country;Japan |
| Language;English |
| Abstract;The analysis of tetracycline, oxytetracyline, chlortetracycline and doxycycline by high-performance liquid chromatography with pulsed amperometric detection using an anodized boron-doped diamond thin film (BDD) electrode is originally reported. The analyses were carried out using the mobile phase, phosphate buffer (0.01 M, pH 2.5)-acetonitrile (80:20; v/v), on a C18 column (250 * 4.6 mm i.d., 5 .MU.m) at a flow rate of 1.0 mL/min. The optimal PAD waveform parameters at the anodized BDD were 1.5 V (versus Ag/AgCl) detection potential (Edet) for 290 ms (200 ms delay time and 90 ms integration time), 2.0 V (versus Ag/AgCl) oxidation potential (Eoxd) for 200 ms oxidation time (toxd) and 0.4 V (versus Ag/AgCl) reduction potential (Ered) for 200 ms reduction time (tred). The proposed method showed the simultaneous determination of tetracycline, oxytetracyline, chlortetracycline and doxycycline with a linear range of 0.1-100 .MU.g/mL, detection limits of 0.05-0.1 .MU.g/mL and recoveries of 70.8-96.0%. The application of this method to real samples was demonstrated and validated using a shrimp sample. (author abst.) |
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