A case of anomaly in enzyme examination by ALP-combining immunoglobulin.
|
Accession number;05A0266889
|
| Title;A case of anomaly in enzyme examination by ALP-combining immunoglobulin. |
| Author;
HORII KOJI
(Sch. of Med., Keio Univ.)
|
Journal Title;Modern Medical Laboratory
|
Journal Code:Z0084B
|
ISSN:0301-2611
|
|
VOL.33;NO.2;PAGE.150-151(2005)
|
| Figure&Table&Reference;FIG.3, REF.1 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The abnormal activity recognized under serum isoenzyme analysis is sometimes caused by enzyme-combining immnoglobulin. The contrivance on the analysis of the appeared abnormal image (alkaline phosphatase (ALP)-combining immunoglobulin) is described. To begin with, the fact that the image of immunofixation after electrophoresis and the stained image of abnormal activity band do not agree, and that there are many cases in which L-chain is undetectable, were questioned. By a study using right-angled electophoresis, it was shown that the combination dissociated in the electrophoresis and by the reaction with antiserum. It was confirmed that in the electrophoretic image, true abnormal fraction and apparent abnormal image exist, and the method to remove protein after the reaction with antiserum is with a problem as a method of identification. It was a question that there were some cases in which the normal fraction disappeared, but it was estimted to be due to the combination of all ALP. By producing the diluted series from the serum in the case and adding ALP, it was shown that the combining ALP come to saturate. From this fact, the existence of un-combined immunoglobulin with combining capacity in the serum was proven. The quantitization of ALP-combining immunoglobulin became also possible. |
|
|
|
Related Articles;
|
|
