Search for early diagnosis marker of intractable cancer.
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Accession number;05A0266895
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| Title;Search for early diagnosis marker of intractable cancer. |
| Author;
HONDA KAZUFUMI
(National Cancer Center Res. Inst., JPN)
YAMADA TETSUSHI
(National Cancer Center Res. Inst., JPN)
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Journal Title;Modern Medical Laboratory
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Journal Code:Z0084B
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ISSN:0301-2611
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VOL.33;NO.2;PAGE.172-174(2005)
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| Figure&Table&Reference;FIG.4, REF.2 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The principle of SELDI-TOF-MS (surface-enhancement laser deionization mass spectrometry) and the search for a marker using this method are described. SELDI-TOF-MS is an analytical method combining a protein chip, a metal plate with a surface modified to catch proteins, with time-of-flight mass spectrometer. When the blood is analyzed, it is fractioned and simplified in advance and put on a protein chip in a minute amount to perform affinity chromatography. Mass spectrometric analysis of the protein caught on the chip is quantitatively performed, and quantitatively visualized as a waveform. Cancer-specific protein is searched from the wave peaks and utilized as a marker. Since it is difficult to distinguish only with a single peak, practically, a mechanical learning algorithm is utilized. Disease group and control group are prepared as a learning set and each peak pattern is memorized in a computer, and the data set contributing to these patterns is extracted. If this is defined strictly, the diagnosis of cancer is possible at a high discriminating rate. |
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