Effects of Immunoglobulin on Murine Myocarditis Caused by Influenza A Virus: Experimental Study
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Accession number;05A0562160
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| Title;Effects of Immunoglobulin on Murine Myocarditis Caused by Influenza A Virus: Experimental Study |
| Author;
KISHIMOTO CHIHARU
(Kyodai Daigakuin'igakukenkyuka Junkankinaika)
HIRAOKA YUJI
(Toyama Med. and Pharm. Univ.)
TAKADA HITOSHI
(Toyama Med. and Pharm. Univ.)
KUROKAWA MASAHIKO
(Toyamaiyakudai I Uirusugaku)
OCHIAI HIROSHI
(Toyamaiyakudai I Uirusugaku)
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Journal Title;J Cardiol
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Journal Code:Y0264A
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ISSN:0914-5087
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VOL.45;NO.6;PAGE.247-255(2005)
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| Figure&Table&Reference;FIG.1, TBL.4, REF.22 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Objectives. Influenza A viruses play the largest role in the worldwide epidemiology of infectious diseases. We examined the effects of intact type and F(ab')2 type of immunoglobulin preparations on murine influenza A virus myocarditis in mice. Methods and Results. In vitro study showed that intact type and F(ab')2 type of immunoglobulin preparations had antiviral activities against many substrains of influenza A virus and other cardiotropic viruses, and that dose-dependent suppression of an influenza A virus (NWS type) was demonstrated by the treatment of both intact immunoglobulin and F(ab')2 fragments of immunoglobulin. The dose inhibiting 50% of plaques was same between intact type and F(ab')2 type (both 0.0002 mg/dl). Intact immunoglobulin, but not F(ab')2 fragments of immunoglobulin, suppressed serum macrophage inflammatory protein-2 (MIP-2) production in influenza A virus infected macrophages in vitro, which is a murine counterpart of interleukin-8. This suppression of MIP-2 production by intact immunoglobulin treatment was blocked by a specific Fc receptor (Fc.GAMMA.III/II receptor) antibody pretreatment. Intact immunoglobulin (1g/kg/day) or F(ab')2 fragments of immunoglobulin (1g/kg/day) were administered to the virus-inoculated A/J mice intraperitoneally daily, starting simultaneously with virus inoculation (Experiment I) and 2 days after the virus inoculation (Experiment II), until 10th days after virus inoculation. In Experiment I, survival was higher in treated[intact(100%, 20/20), and F(ab')2(100%, 20/20)]than in control(25%, 5/20)mice; intact type and F(ab')2 type immunoglobulin administration completely suppressed the development of myocarditis. In Experiment II, survival rate was significantly higher(75%, 15/20) and myocarditis was less severe in intact immunoglobulin treated mice, but not in F(ab')2 fragment treated mice (60%, 12/20), than in untreated mice (35%, 7/20).... (author abst.) |
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