PLAGIOCHILA SPINULOSA (DICKS.) DUMORT. (PLAGIOCHILACEAE, HEPATICOPHYTINA) IN MADEIRA ISLAND-MORPHOLOGICAL, PHYTOCHEMICAL, AND MOLECULAR EVIDENCE
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Accession number;05A0766123
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| Title;PLAGIOCHILA SPINULOSA (DICKS.) DUMORT. (PLAGIOCHILACEAE, HEPATICOPHYTINA) IN MADEIRA ISLAND-MORPHOLOGICAL, PHYTOCHEMICAL, AND MOLECULAR EVIDENCE |
| Author;
SIM-SIM MANUELA
(Universidade De Lisboa, Lisboa, Prt)
STECH MICHAEL
(Freie Univ. Berlin, Berlin, Deu)
ESQUIVEL M. GLORIA
(Isa, Lisboa, Prt)
FIGUEIREDO A. CRISTINA
(Universidade De Lisboa, Lisboa, Prt)
COSTA MONYA M.
(Universidade De Lisboa, Lisboa, Prt)
BARROSO JOSE G.
(Universidade De Lisboa, Lisboa, Prt)
PEDRO LUIS G.
(Universidade De Lisboa, Lisboa, Prt)
FONTINHA SUSANA
(Servico Do Parque Natural Da Medeira, Madeira, Prt)
LOBO CARLOS
(Jardim Botanico Da Madeira, Madeira, Prt)
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Journal Title;J Hattori Bot Lab
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Journal Code:Y0255A
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ISSN:0073-0912
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VOL.;NO.98;PAGE.131-147(2005)
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| Figure&Table&Reference;FIG.4, TBL.1, REF.43 |
| Pub. Country;Japan |
| Language;English |
| Abstract;The occurrence of Plagiochila spinulosa in Madeira Island was questionable according to previous authors and our revisions of herbarium specimens collected in the Madeiran laurel forest, which belong to either P. bifaria, P. stricta, or the endemic P. maderensis. Recent fieldwork carried out above the edge of the laurel forest between 1300 and 1400m directed our attention to some Plagiochila populations that presented a distinct appearance in the field. A first morphological investigation revealed that these populations could belong to P. spinulosa. In addition to morphological characters observed with light and scanning electron microscopy, the essential oil composition and sequences of the ITS1-5.8S-ITS2 region of the nuclear ribosomal DNA are compared between the putative P. spinulosa and further Plagiochila species of sect. Arrectae, including maximum parsimony and maximum likelihood analyses of the molecular data. Essential oils isolated by distillation-extraction from two collections of the putative P. spinulosa were analysed by GC and GC-MS. Sabinene (19-27%) was the dominant component of both samples, in contrast to all other previously analysed Plagiochila species. In the molecular trees a clade is observed that comprises P. spinulosa from Belgium and the UK, and three analysed specimens of the putative P. spinulosa from Madeira Island. This clade is well distinguished from the other species of sect. Arrectae with 75-81% bootstrap support. The morphological, phytochemical and molecular results thus confirm the presence of Plagiochila spinulosa in Madeira Island, which seems to be confined to communities in the mesotemperate hyper-humid bioclimatic zone above the limits of the laurel forest. (author abst.) |
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