Identification of four species of the Anopheles hyrcanus complex (Diptera: Culicidae) found in Korea using species-specific primers for Polymerase Chain Reaction Assay
|
Accession number;05A0981352
|
| Title;Identification of four species of the Anopheles hyrcanus complex (Diptera: Culicidae) found in Korea using species-specific primers for Polymerase Chain Reaction Assay |
| Author;
REE HAN-IL
(Yonsei Univ., Seoul, Kor)
YONG TAI-SOON
(Yonsei Univ., Seoul, Kor)
HWANGU UI-WOOK
(Yonsei Univ., Seoul, Kor)
HWANGU UI-WOOK
(Kyungpook National Univ., Taegue, Kor)
|
Journal Title;Medical Entomology and Zoology
|
Journal Code:Z0441B
|
ISSN:0424-7086
|
|
VOL.56;NO.3;PAGE.201-205(2005)
|
| Figure&Table&Reference;FIG.2, TBL.1, REF.16 |
| Pub. Country;Japan |
| Language;English |
| Abstract;For identification of four sibling species of the Anopheles hyrcanus complex found in Korea, the 5.8 rDNA-ITS2-28S rDNA region of each species was sequenced and the species-specific primers were designed. The amplified PCR products obtained from each species were analyzed by electrophoresis on 3% agarose gel. The result showed a single species-specific band, i.e. 559 bp, 432 bp, 322 bp and 192 bp for An. sinensis, An. sp., An. lesteri and An. pullus, respectively. In conclusion, the species-specific PCR primers designed from ITS2 variable regions functioned successfully and specifically, and can be applied as a useful tool for identifying species of the Hyrcanus complex found in Korea. (author abst.) |
|
|
|
Related Articles;
|
|
