Improvement in Cryopreservation of Bovine Immature Oocytes by Vitrification with Step-wise Exposure to Cryoprotectants
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Accession number;05A1044256
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| Title;Improvement in Cryopreservation of Bovine Immature Oocytes by Vitrification with Step-wise Exposure to Cryoprotectants |
| Author;
ABE YASUYUKI
(Tohoku Univ., Graduate School of Agricultural Sci., JPN)
SATO EIMEI
(Tohoku Univ., Graduate School of Agricultural Sci., JPN)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.23;NO.;PAGE.13-17(2005)
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| Figure&Table&Reference;FIG.1, TBL.4, REF.10 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The present study was conducted to demonstrate effects of sugars and protocol of exposure in vitrification on subsequent in vitro maturation and development in bovine immature oocytes after cryopreservation using nylon mesh. Immature bovine cumulus-oocytes complexes (COCs) were collected from ovaries and subjected to the experiments. Before vitrification, COCs were exposed to the cryoprotectant, which was composed of 40% ethylene glycol, 18% Ficoll-70, and either of 0.3 M sucrose (EFS), 0.3 M trehalose (EFT) or 0.3 M raffinose (EFR), either by single step or in a stepwise way. The maturation and cleavage rates in the exposure with EFS were significantly higher (P<0.05) compared with the corresponding rates in EFT or EFR. While, in the stepwise exposure, few abnormalities were observed, and maturation and cleavage rates were significantly higher (P<0.05) compared with the corresponding rates in the single step exposure. The cleaved embryos derived from the stepwise exposure to EFS developed to blastocysts. In conclusion, our results suggest that sucrose as a cryoprotectant accompanied with stepwise exposure may have advantage in vitrification of large numbers of bovine immature oocytes, resulting in yield of blastocysts following in vitro embryo production. (author abst.) |
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