An Analysis of Spatial and Temporal Gene Expression Profiles by a Custom-made cDNA Utero-placental Microarray
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Accession number;05A1044257
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| Title;An Analysis of Spatial and Temporal Gene Expression Profiles by a Custom-made cDNA Utero-placental Microarray |
| Author;
HASHIZUME KAZUYOSHI
(Iwate Univ., Faculty of Agriculture, JPN)
TAKAHASHI TOORU
(National Inst. Agrobiological Sci.)
KOSAKA TETSUYA
(Shizuoka Univ., Fac. Agriculture, JPN)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.23;NO.;PAGE.18-24(2005)
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| Figure&Table&Reference;FIG.4, TBL.1, REF.19 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;This study reports the identification of specific member of bovine utero-placental genes by a custom-made cDNA microarray. After k-means clustering analysis, we found three genes which expressed in placenta with progress of gestation period and the expression profiles were closely related to placental lactogen (PL) which is a specific molecule in trophoblast cell in bovine. One of three genes was identified as a new member of bovine prolactin-related proteins (PRPs) after sequence of a full-length cDNA. We named it as bPRP-VII, and their quantitative and localized expressions were examined in the placenta. A full-length bPRP-VII cDNA was cloned with a 717-nucleotide open-reading-frame corresponding to a protein of 238 amino acids. The predicted amino acid sequence shares 63% homology with bPRP-I and 70% with bPRP-VI. bPRP-VII has eight cysteine residues with four disulfide bonds, which is more abundant than that of other bPRPs. RT-PCR detected bPRP-VII only in the placenta. In the placenta, mRNA was expressed in the cotyledon and intercotyledonary tissues throughout gestation. Quantitative real-time RT-PCR analysis exhibited a high expression of bPRP-VII mRNA in the fetal membrane at Day 27 of gestation. In the placentome on Day 60 of gestation, in situ hybridization analysis evidenced bPRP-VII mRNA in binucleate cells. Expression profiles and localization were similar to those of bPRP-I. Although the functional data remain to be examined, a new member of the bPRP-VII gene may take on an important functional role in implantation same as bPRP-I. This molecule was identified as a new member of PRP since Linda Schuler group found PRP-IV 14 years ago. This finding depends on the new technology, microarray and bioinformatics, and the present study suggests that these technologies bring to a new site for study of bovine placental functions. (author abst.) |
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