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Accession number;05A1044259
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| Title;Development of an Effective System to Control Sperm Penetrability for Artificial Insemination and In Vitro Fertilization: Utilization of Oxidant and Anti-Oxidant |
| Author;
FUNAHASHI HIROAKI
(Okayama Univ., Graduate School of Natural Sci. and Technol., JPN)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.23;NO.;PAGE.32-38(2005)
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| Figure&Table&Reference;FIG.3, TBL.2, REF.5 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;This research was undertaken to develop an effective system to regulate sperm functions for artificial insemination and in vitro fertilization. In the first experimental series, effect of beta-mercaptoethanol (bME) on sperm functions of freshly ejaculated boar spermatozoa was examined. Ejaculated boar spermatozoa were washed and resuspended in the absence or presence of bME in fertilization medium containing 5 mM caffeine. In this experiment, it has been found that the presence of bME (at 50 .MU.M or more) prevents stimulate effect of caffeine on capacitation and spontaneous acrosome reaction of boar spermatozoa. The second experiment series was to determine the effect of bME on sperm penetrability in vitro of boar spermatozoa. Spermatozoa were co-incubated transiently (for 10 min) in the absence or presence of 50 .MU.M bME in modified Medium 199 containing 5 mM caffeine and then continued an additional culture in the absence or presence of 50 .MU.M bME in modified Medium 199 till 8 h after insemination. Results of these experiments have demonstrated that the presence of bME during co-culture and additional culture reduced the incidence of sperm penetration and that the presence of bME only during additional culture increased the incidence of monospermic penetration. In the third experiment, effect of hydrogen peroxide on sperm function was determined. Spermatozoa were cultured in the absence or presence of hydrogen peroxide in caffeine-free modified Medium 199. As the result, it was found that hydrogen peroxide stimulated sperm capacitation but not affect the acrosome reaction. This research project has succeeded to regulate penetrability of boar spermatozoa by using a reducing agent and an oxidative. (author abst.) |
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