Simplified Assay for the Reproductive Performance of Male Japanese Black Cattle by Quantitative Analysis of Sperm Phosphorylated Proteins
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Accession number;05A1044260
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| Title;Simplified Assay for the Reproductive Performance of Male Japanese Black Cattle by Quantitative Analysis of Sperm Phosphorylated Proteins |
| Author;
HARAYAMA HIROSHI
(Kobe Univ., Graduate School of Sci. and Technol., JPN)
KURODA KEN
(Kobe Univ., Graduate School of Sci. and Technol., JPN)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.23;NO.;PAGE.39-44(2005)
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| Figure&Table&Reference;FIG.1, REF.7 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The aim of this study was to examine validity of quantitative analysis of cAMP-dependent serine/threonine-phosphorylated sperm proteins for evaluation of reproductive performance of male Japanese Black cattle. Frozen semen from eight subfertile and four fertile bulls were thawed, washed and then resuspended in a Hepes-buffered BO medium containing a cAMP analog cBiMPS (0.2 mM). The samples were subjectively assessed for sperm motility and then incubated at 38.5 .DEG.C. for 180 minutes. After the incubation, the sperm samples were used for Western blotting and indirect immunofluorescence to detect serine/threonine-phosphorylated sperm proteins. Approximately 70% of the spermatozoa were motile in all the samples from eight subfertile and four fertile bulls. However, the percentages of progressively motile spermatozoa were lower in the samples from four subfertile bulls (7-12%) than in those from four fertile bulls (an average percentage: 37%). When the spermatozoa from the fertile bulls were treated with the cAMP analog, four major serine/threonine-phosphorylated proteins (220 kDa, 82 kDa, 61 kDa and 42 kDa) appeared in the flagella. However, these phosphorylated proteins slightly increased in the spermatozoa from the above-mentioned subfertile bulls. These findings suggest that low progressive motility and low flagellar PKA activity may be causal factors for subfertility of male Japanese Black cattle, and that quantitative analysis of cAMP-dependently serine/threonine-phosphorylated proteins is valid for evaluation of bull reproductive performance. (author abst.) |
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