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Accession number;05A1044261
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| Title;Studies on the Production of Somatic Cell Cloned Pigs |
| Author;
TSUNODA YUKIO
(Kinki Univ., Faculty of Agriculture, JPN)
KATO YOKO
(Kinki Univ., Faculty of Agriculture, JPN)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.23;NO.;PAGE.45-47(2005)
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| Figure&Table&Reference;REF.5 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Since the first successful production of somatic cell-cloned piglets in 2000, several laboratories have produced porcine clones. However, cryopreservation of cloned porcine embryos has not been done. Since it was reported that delipation from porcine embryos is effective for freezing of porcine embryos, we examined the effect of delipation on the survival of porcine somatic cell nuclear-transferred blastocysts after freezing and thawing. Blastocysts developed from enucleated oocytes receiving fetal somatic cells were cryopreserved by vitrification using the minimum volume of freezing medium and the in vitro developmental potential of frozen-thawed embryos were examined. Prior to freezing, the cytoplasmic lipid droplets of embryos at 2-cell stage were mechanically removed after centrifugation. Survival rates of early and expanded blastocysts were significantly improved by delipation (50% vs 0% for early blastocysts and 72% vs 12% for expanded blastocysts). The present study clearly demonstrated that porcine somatic cell-cloned embryos can be effectively cryopreserved. (author abst.) |
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