Establishment of the Technology of Freezing Boar Semen by Inseminating a Small Number of Sperm
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Accession number;05A1044262
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| Title;Establishment of the Technology of Freezing Boar Semen by Inseminating a Small Number of Sperm |
| Author;
YAMAGUCHI SHOICHIRO
(Fukuokakennogyosogoushi)
MURAKAMI TETSUYA
(Fukuokakennogyosogoushi)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.23;NO.;PAGE.48-53(2005)
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| Figure&Table&Reference;FIG.3, TBL.2, REF.9 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;The purpose of the study was to establish a practical technology of freezing boar semen with normal pregnancy rate and litter size by inseminating a small number of spermatozoa. The first test: 1) Sows were inseminated twice after hCG treatment, using the intrauterine insemination catheter with frozen-thawed semen (20*10'8' cells per dose), extended with Modena solution or modified BTS solution (BCC solution) containing caffeine and CaCl2. The resulting conception rate was 40% (2/5) and 80% (4/5), farrowing rate was 20% (1/5) and 60% (3/5) and litter size was 6 and 10.3 piglets for Modena solution and BCC solution, respectively. 2) Sows were inseminated once at 40 h after hCG treatment using the intrauterine insemination catheter with Large White (60% motile) and Duroc (30% motile) frozen-thawed semen (20*10'8' cells) diluted with BCC solution. The resulting conception rate was 100% (4/4) and 25% (1/4), farrowing rate was 100% (4/4) and 25% (1/4) and litter size was 7.8 and 11 piglets for Large White and Duroc, respectively. In order to aim at utilization with 0.5ml straw, it was examined for the purpose of freezing semen in high concentration and raising convenience as the second examination. (1) Post-thaw sperm motility was lower in spermatozoa treated by the routine procedure when concentrating (1.4-2times) and freezing spermatozoa, but the concentration of 1.2 times had no effect on sperm motility. (2) Post-thaw sperm motility was much lower in spermatozoa treated by the routine procedure when concentrating (1.5-2times), after centrifuging at 5.DEG.C. and freezing spermatozoa. (author abst.) |
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