Study of GI-dismotility in Enteritis and Colitis
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Accession number;05A1044265
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| Title;Study of GI-dismotility in Enteritis and Colitis |
| Author;
SATO KOICHI
(Yamaguchi Univ., Fac. Agriculture, JPN)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.23;NO.;PAGE.65-69(2005)
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| Figure&Table&Reference;FIG.3, REF.7 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Although the motility disorders of the gastro-intestinal tract are well found in patients with inflammatory bowel diseases not only in humans but also in large animals, the mechanism of the dysmotility has not yet been cleared. For the livestock management, the motility disorder is a big problem because of the loss of productivity. In order to elucidate the mechanism of the motility disorder, we used the inflamed distal colon isolated from dextran sodium sulphate-induced ulcerative colitis model mouse (DSS-treated mouse). In the DSS-treated mouse, morphological analysis indicated that the mucosa of distal colon was ulcerated and both of the submucosal layer and the smooth muscle layer became thick. Carbachol-induced contraction in vitro was reduced in DSS-treated mouse colon. Preincubation with l-NMMA (non-selective NO synthase inhibitor), indomethacin (non-selective COX inhibitor) or glibenclamide (ATP-sensitive potassium channel inhibitor) did not modify the reduced carbachol-induced contraction in DSS-treated mouse colon. In semi-quantitative RT-PCR experiments and Western blotting analysis, neither of the expression level of and M3 muscarinic receptor mRNA and protein of M3 muscarinic receptor has changed. The Ca'2+'-sensitivity of contractile elements in smooth muscle cells, as assessed in the .BETA.-escin permeabilized colonic strip stimulated by carbachol with GTP or GTP-.GAMMA.S, was significantly reduced. Expression of proteins involving in Ca'2+'-sensitization of contraction in smooth muscle cells (rhoA, ROCK1 and ROCK2) were not different between control and DSS-treated mouse colon. However, the expression level of CPI-17, the functional proteins involved in the smooth muscle myosin phosphatase activity, was significantly decreased in the DSS-treated mouse colon.... (author abst.) |
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