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Accession number;05A1044266
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| Title;Cultivation of Lawsonia intracellularis and Diagnosis of Proliferative Enteropathy in Pig |
| Author;
WATARAI MASAHISA
(Obihiro Univ. of Agric. and Vet. Med.)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.23;NO.;PAGE.70-74(2005)
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| Figure&Table&Reference;FIG.3, REF.3 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Lawsonia intracellularis is an obligate intracellular pathogenic bacterium that causes proliferative enteropathy in domestic and experimental animals. We cultivated L. intracellularis from homogenized intestine from a rabbit infected with L. intracellularis by IEC-18 cells. Many intracellular bacteria were recognized with serum against synthetic peptides in the Lawsonia surface antigen (LsaA) by immunohistochemistry. Staining of an ileum section from infected rabbits by anti-LsaA serum also showed clusters of many bacteria in the apical cytoplasm of enterocytes. The synthetic peptides in LsaA reacted with serum infected with L. intracellularis, which was tested by enzyme-linked immunosorbent assay. These results suggest that synthetic peptides in LsaA and the antiserum against the peptides might be useful to diagnose for proliferative enteropathy. The detection of L. intracellularis in clinical and environmental samples is necessary for the diagnosis of infection and epidemiological investigations. For the detection of L. intracellularis in fecal samples, we developed an immunological method using iminunomagnetic separation and ATP bioluminescence. Magnetic beads were coated with an anti-Lawsonia surface antigen (LsaA) antibody in order to capture the L. intracellularis in fecal samples from infected rabbits and the bacteria captured by the immunomagnetic beads were tried to detect by means of ATP bioluminescence. Our results showed that L. intracelluraris was detected by immunomagnetic separation of bacteria-holding magnetic beads and ATP-based bioluminescence, and suggested that our methods could be useful for the diagnosis of proliferative enteropathy. (author abst.) |
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