| Abstract;Poultry meat and eggs contaminated with Salmonella enterica serovar Enteritidis (SE) are common sources of food poisoning worldwide. Vaccine trials against Salmonella infection in poultry industry were not ineffective. However, while live vaccine against Salmonella is available in some of foreign countries, it is not allowed to use it in Japan and also the safety concerned for the individual is not enough. It is expected that one of the most possible candidates is a recombinant sub-unit vaccine. For development of Salmonella recombinant vaccine, it is of great advantage to use chicken monoclonal antibodies against Salmonella for antigenic analysis. We attempted to prepare chicken monoclonal antibodies (mAb) against SE. Three monoclonal antibodies designated Al, A2 and A3 were selected. Western blotting analyses revealed that all three mAbs reacted with the 43-kDa molecular weight band of SE, S. Hadar, S. Montevideo (SM), S. Heidelberg (She) antigens, 44-kDa of SE, SM, S. Infantis, She antigens. Furthermore, these epitopes appeared to be periodate insensitive. These results indicate that the target antigen recognized by these chicken mAbs might have a shared epitope, which is present on many Salmonella serotypes. (author abst.) |
