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Accession number;05A1044273
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| Title;Physiological Role of Ghrelin on Proliferation and Differentiation of Preadipocyte |
| Author;
ROH SANG-GUN
(Shinshu Univ., Fac. Agriculture, JPN)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.23;NO.;PAGE.111-114(2005)
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| Figure&Table&Reference;FIG.1, REF.1 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Ghrelin and growth hormone secretagogues receptor (GHS-R or ghrelin receptor) have been reported as one of the factors of adipogenesis in adipocytes. To investigate the involvement of ghrelin and GHS-R in adipocyte, we evaluated the effect of the GHS-R antagonist [D-Lys-3]-GHRP-6 (His-D-Trp-D-Lys-Trp-D-Phe-Lys-NH2), on the process of adipogenesis in ovine adipocytes. [D-Lys-3]-GHRP-6 (10'-7'mol/l) significantly inhibited adipogenic differentiation of ovine preadipocytes prepared from adipose tissues. The level of PPAR-.GAMMA.2 mRNA, an adipogenic marker, decreased during the differentiation of adipocytes treated with [D-Lys-3]-GHRP-6 for 10 days. Ghrelin stimulated adipogenesis, also causing increment of GPDH and up-regulation of PPAR-.GAMMA.2. Further, the anti-lipolytic effect of ghrelin was attenuated by treatment with [D-Lys-3]-GHRP-6 in the isolated adipocytes. Overall, our results highlight a role of GHS-R in adipogenesis that can be blocked by treatment with [D-Lys-3]-GHRP-6. (author abst.) |
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