Studies on Skeletal Muscle Satellite Cell Quiescence In Vitro
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Accession number;05A1044280
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| Title;Studies on Skeletal Muscle Satellite Cell Quiescence In Vitro |
| Author;
TATSUMI RYUICHI
(Kyushu Univ., School of Agriculture, JPN)
SHIRATSUCHI SEIICHI
(Kyushu Univ., Graduate School of Bioresource and Bioenvironmental Sciences, JPN)
KATSUKI YOSHITAKA
(Kyushu Univ., Graduate School of Bioresource and Bioenvironmental Sciences, JPN)
HARUNO ATSUSHI
(Kyushu Univ., Graduate School of Bioresource and Bioenvironmental Sciences, JPN)
IKEUCHI YOSHIHIDE
(Kyushu Univ., School of Agriculture, JPN)
SEKIGUCHI TAKESHI
(Itoraifusaiensu)
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Journal Title;Shokuniku ni kansuru Josei Kenkyu Chosa Seika Hokokusho
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Journal Code:X0296A
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ISSN:
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VOL.23;NO.;PAGE.148-153(2005)
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| Figure&Table&Reference;FIG.2, REF.17 |
| Pub. Country;Japan |
| Language;Japanese |
| Abstract;Postnatal muscle growth and regeneration rely on molecular events responsible for activation and quiescence of satellite cells. Recent studies demonstrated that hepatocyte growth factor (HGF) triggers satellite cell activation entry into the cell cycle in response to mechanical perturbation (Allen et al., 1995; Tatsumi et al., 1998, 2001, 2002a, b), and that the subsequent expression of myostatin (GDF 8) signals the cell quiescence (McPherron et al., 1997; McCroskery et al., 2003). Mechanisms responsible for coordinating expression of myostatin after an appropriate time-lag for satellite cell activation and proliferation are not clear. Here we address the possible role of the HGF in the quiescence through a negative feedback mechanism following satellite cell activation. Experiments were conducted with primary cultures of rat quiescent satellite cells. Activation was stimulated for 24 hr by 2.5 ng/ml human recombinant HGF; this concentration has been shown to peak activation. Cultures were then incubated in the media containing higher concentrations of HGF (2.5-500 ng/ml) for the next 24 hr. HGF concentrations greater than 10 ng/ml decreased the activation index down to a level comparable to 24-hr control cultures not receiving HGF. The high level HGF treatment did not impair the cell viability, and cells could be re-activated by lowering HGF concentrations to 2.5 ng/ml in media. The inactivation of satellite cells could be rescued by increasing concentrations of anti-myostatin neutralizing antibody in medium up to a peak level comparable to activated control culture. Also, myostatin protein expression and secretion were demonstrated by immunocytochemical localization and by western blots of cell lysate and conditioned medium from high level HGF-treated cells. These results indicate that HGF could induce satellite cell quiescence by stimulating expression and secretion of myostatin in vitro.... (author abst.) |
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