Inhibitory Effects of Ruta graveolens L. Extract on Guinea Pig Liver Aldehyde Oxidase
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Accession number;06A0025391
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| Title;Inhibitory Effects of Ruta graveolens L. Extract on Guinea Pig Liver Aldehyde Oxidase |
| Author;
SAIEED PIROUZPANAH
(School Of Health And Nutrition, Tabriz Univ. Of Medical Sciences)
REZA RASHIDI MOHAMMAD
(Drug Applied Res. Center, Tabriz Univ. Of Medical Sciences)
ABBAS DELAZAR
(Drug Applied Res. Center, Tabriz Univ. Of Medical Sciences)
SEYYEDVALI RAZAVIEH
(School Of Health And Nutrition, Tabriz Univ. Of Medical Sciences)
ALIASGHAR HAMIDI
(School Of Pharmacy, Tabriz Univ. Of Medical Sciences)
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Journal Title;Chem Pharm Bull
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Journal Code:G0504A
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ISSN:0009-2363
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VOL.54;NO.1;PAGE.9-13 (J-STAGE)(2006)
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| Figure&Table&Reference;FIG.2, TBL.3, REF.51 |
| Pub. Country;Japan |
| Language;English |
| Abstract;Ruta graveolens L. is a flavonoid-containing medicinal plant with various biological properties. In the present study, the effects of R. graveolens extract on aldehyde oxidase, a molybdenum hydroxylase, are investigated. Aldehyde oxidase was partially purified from liver homogenates of mature male guinea pigs by heat treatment and ammonium sulphate precipitation. The total extract was obtained by macerating the aerial parts of R. graveolens in MeOH 70% and the effect of this extract on the enzyme activity was assayed using phenanthridine, vanillin and benzaldehyde as substrates. Quercetin and its glycoside form, rutin were isolated, purified and identified from the extract and their inhibitory effects on the enzyme were investigated. R. graveolens extract exhibited a high inhibition on aldehyde oxidase activity (89-96%) at 100 .MU.g/ml which was comparable with 10 .MU.M of menadione, a specific potent inhibitor of aldehyde oxidase. The IC50 values for the inhibitory effect of extract against the oxidation of benzaldehyde, vanillin and phenanthridine were 10.4, 10.1, 43.2 .MU.g/ml, respectively. Both quercetin and rutin at 10 .MU.M caused 70-96% and 27-52% inhibition on the enzyme activity, respectively. Quercetin was more potent inhibitor than rutin, but both flavonols exerted their inhibitory effects mostly in a linear mixed-type. (Author abst.) |
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